ProteanFect Transfection Reagent is designed to efficiently transfect even the most challenging cell types, ensuring superior performance across a broad range of cells. It also excels at delivering high molecular weight nucleic acids or co-transfecting multiple nucleic acids with exceptional efficiency. For research use only.
| Number | Standard | Price | Stock |
|---|---|---|---|
| PT0101 | 0.1 mL | In quiry | In stock |
| PT0102 | 0.3 mL | In quiry | In stock |
| PT0103 | 0.5 mL | In quiry | In stock |
| PT0104 | 1 mL | In quiry | In stock |
| PT0105 | 5 mL | In quiry | In stock |
The ProteanFect Transfection Kit is shipped on dry ice and should be stored at -20°C until use. The kit includes positive control samples, such as mEFP and plasmid DNA (pDNA) encoding green fluorescent protein (EGFP). After opening, to ensure optimal performance, the components must be stored in the conditions specified in the table below.
| Element | Storage |
|---|---|
| Reagent A | 2-8°C (Store at 2-8°C after opening) |
| Reagent B | -20°C (Avoid repeated freezing and thawing) |
| EGFP mRNA (1 μg / μL) | -20°C (Avoid repeated freezing and thawing) |
| EGFP pDNA (0.5 μg / μL) | -20°C (Avoid repeated freezing and thawing) |
Transported on Dry Ice
Successfully transfected cell types using ProteanFect Transfection Kit include:
| Cell lines | Compatible nucleic acids for transfection |
|---|---|
| Jurkat T (human T lymphoblastic leukemia cell) | pDNA, mRNA, siRNA |
| LX-2 (human hepatic stellate cell) | pDNA, mRNA, siRNA |
| HepG2 (human liver tumor cell) | pDNA, mRNA, siRNA |
| THP-1 (human acute monocytic leukemia cell) | mRNA, siRNA |
| Raji (human Burkitt's lymphoma cell) | mRNA, siRNA |
| K562 (human chronic myeloid leukemia cell) | pDNA, mRNA, siRNA |
| MOLT-16 (human T lymphoblastic leukemia) | mRNA, siRNA |
| U937 (human lymphoma cell) | mRNA, siRNA |
| HEK-293 (human embryonic kidney cell) | pDNA, mRNA, siRNA |
| MC38 (mouse colon cancer cell) | mRNA, siRNA |
| RAW264.7 (mouse mononuclear macrophage leukemia cell) | mRNA, siRNA |
| LLC (Mouse Lewis lung cancer cell) | pDNA, mRNA, siRNA |
| C2C12 (mouse myoblast) | pDNA, mRNA, siRNA |
